3-alpha,7-alpha-dihydroxy-5-beta-cholestanate—CoA ligase

In enzymology, a 3α,7α-dihydroxy-5β-cholestanate—CoA ligase (EC 6.2.1.28) is an enzyme that catalyzes the chemical reaction

ATP (25R)-3alpha,7alpha-dihydroxy-5beta-cholestan-26-oate CoA ${\displaystyle \rightleftharpoons }$ AMP diphosphate (25R)-3alpha,7alpha-dihydroxy-5beta-cholestanoyl-CoA

The 3 substrates of this enzyme are ATP, (25R)-3alpha,7alpha-dihydroxy-5beta-cholestan-26-oate, and CoA, whereas its 3 products are AMP, diphosphate, and (25R)-3alpha,7alpha-dihydroxy-5beta-cholestanoyl-CoA.

This enzyme belongs to the family of ligases, specifically those forming carbon-sulfur bonds as acid-thiol ligases. The systematic name of this enzyme class is (25R)-3alpha,7alpha-dihydroxy-5beta-cholestan-26-oate:CoA ligase (AMP-forming). Other names in common use include 3alpha,7alpha-dihydroxy-5beta-cholestanoyl coenzyme A synthetase, DHCA-CoA ligase, and 3alpha,7alpha-dihydroxy-5beta-cholestanate:CoA ligase (AMP-forming). This enzyme participates in bile acid biosynthesis.

References

• Prydz K, Kase BF, Björkhem I, Pedersen JI (August 1988). "Subcellular localization of 3 alpha, 7 alpha-dihydroxy- and 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoyl-coenzyme A ligase(s) in rat liver". Journal of Lipid Research. 29 (8): 997–1004. PMID 3183523.